Book Chapters
Peer-Reviewed Publications
Patent and Invention Disclosures
Books Edited
Our Lab Manuals
Book Chapters
- Beverage Industry. In Biomolecular Engineering Solutions for Renewable Specialty Chemicals – Microorganisms, Products, and Processes. Editors: NK Rathinam and RK Sani. Wiley. In Press
- Govil T, Salem DR, and Sani RK. 2021. Bioplastics Production: What Have We Achieved? In Biomolecular Engineering Solutions for Renewable Specialty Chemicals – Microorganisms, Products, and Processes. Editors: NK Rathinam and RK Sani. Wiley. In Press
- Samanta D, Govil T, Saxena P, Thakur P, Narayanan A, and Sani RK. 2021. Extremozymes and Their Applications. In Extremozymes and their Industrial Applications to be published by Springer -Nature. Editors: Prof. Naveen Kumar Arora, Dr. Shekhar Agnihotri, and Dr. Jitendra Mishra. In Press
- Govil T, Salem DR, and Sani RK. 2021. Biorefining of Lignin Wastes: Modularized production of Value-added compounds in Microbial Biotechnology for Renewable and Sustainable Energy. Springer Book. Editors: J. Saini and RK Sani. In Press
- Govil T, Narayanan AS, Salem DR, Sani RK. 2021. Progress in consolidated bioprocessing of lignocellulosic biomass for biofuels and biochemicals, in Microbial Biotechnology for Renewable and Sustainable Energy. Editors: J. Saini and RK Sani. Springer Book. In Press
- Govil T, Solanki S, Hogan Z, Kumar S, Salem DR, and Sani RK. 2021. Understanding the Potential Applications of Biofilms as Industrial “Cell Factories”. Springer Nature Singapore Pte Ltd. (Publisher). Editors: Saini JK and Sani RK. In Press
- Govil T, Salem DR, Syal S and Sani RK. 2021. Understanding the role of a thermophilic genus Geobacillus in the hydrolytic and oxidative degradation of plant biomass: insights from genomics and metagenomic analyses, in Extremophiles: The future prospective in biorefineries. CRC Press. Editor: Pratibha Dheeran and Sachin Kumar.
- Govil T, Sharma W, Salem DR, and Sani RK. 2021. Multi-omics approaches for extremophilic microbial, genetic, and metabolic diversity, In Extreme Environments. Editor: A. Pandey. CRC Press, Taylor & Francis Group.
- Sengor S and Sani RK. 2021. Biogeochemical Interactions of Bioreduced Uranium Nanoparticles, In Microbial Interactions at Nanobiotechnology Interfaces: Molecular Mechanisms and Applications. Editors: NK Rathinam and RK Sani. Wiley. In Press
- Sengor S and Sani RK. 2021. Characterization and Quantification of Mobile Bioreduced Uranium Phases, In Microbial Interactions at Nanobiotechnology Interfaces: Molecular Mechanisms and Applications. Editors: NK Rathinam and RK Sani. Wiley. In Press
- Wang J, Salem DR, and Sani RK. 2020. The microbial polymers produced from methane: Overview of recent progress and new perspectives, In Microbial and Natural Macromolecules: Synthesis and Applications. Editors: S Das and HR Dash. Academic Press, Elsevier. In Press.
- Singh SS, Salem DR, and Sani RK. 2020. Spectroscopy and microscopic techniques for characterization of microbial molecules with major emphasis on Bacterial Nanocellulose, In Microbial and Natural Macromolecules: Synthesis and Applications. Editors: S Das and HR Dash. Academic Press, Elsevier. In Press.
- Samanta D, Govil1 T, Salem DR, Krumholz LR, Gerlach R, Gadhamshetty V, and Sani RK. 2019. Methane Monooxygenases: Their Regulations and Applications in Biofuel Production, In Microbes for sustainable development and Bioremediation. Editors: R. Chandra and RC Sobti. CRC Press, Taylor & Francis Group. Pages 187-206.
- Islam J, Shrestha N, Bathi JR, Sani RK, and Gadhamshetty V. 2020. Surface modification approaches: Electricity generation from Methane in Bioelectrochemical Systems. Eds. P. Kumar and C. Kuppam. Springer. Pages 343-374.
- Govil T, Rathinam NK, Salem DR, and Sani RK. 2019. Taxonomical diversity of extremophiles in the deep biosphere, In Microbial diversity in the genomic era. Eds. S. Das and HR Dash. Elsevier. Pages 631-656.
- Arora R, Sharma NK, Kumar S, and Sani RK. 2018. Lignocellulosic ethanol: feedstocks and bioprocessing. In Bioethanol production from food crops (page 165-185), Ed. Ray RC and Ramachandran S. Elsevier.
- Rathinam NK, Salem D, and Sani RK. 2018. Biofilm Engineering for improving the performance of Microbial Electrochemical Systems. In Microbial Electrochemical Technology: Platform for Fuels, Chemicals and Remediation. Eds. V. Mohan, S. Varjani and A. Pandey. Elsevier.
- Rathinam NK, Salem D, and Sani RK. 2018. Rewiring Extremophilic Electrocatalysis for Production of Biofuels and Value-added Compounds from Lignocellulosic Biomass, In Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power Ed. R.K. Sani and N.K. Rathinam. Springer.
- Wang J, Rathinam NK, Salem D, and Sani RK. 2018. Integrated Consolidated Bioprocessing for Conversion of Lignocellulosic Feedstock to Biofuels and Value-added Bioproducts, In Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power Ed. R.K. Sani and N.K. Rathinam. Springer.
- Rathinam NK and Sani RK. 2018. Bioprospecting of Extremophiles for Biotechnology Applications, In Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. Ed. RK. Sani and R.N. Krishnaraj. Springer, USA.
- Khandeparker R and Sani RK. 2018. Biobutanol Production using Recombinant Microorganisms/Extremophiles. In Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. Ed. RK Sani and R.N. Krishnaraj. Springer, USA.
- Rao R, Sani RK, and Kumar S. 2018. Hydrogen Production from Lignocellulosic Feedstocks using Extremophiles. In Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. Ed. RK Sani and R.N. Krishnaraj. Springer, USA.
- Liew KJ, Lim CC, Chan CS, Wei KY, Salleh MM, Sani RK, Chan KG, and Goh KM. 2018. Direct Cellulase Gene Amplification from Hot Spring Using the Guidance of 16S rRNA Amplicon Metagenomics. In Metagenomics Perspectives, Methods, and Applications. Ed. M. Nagarajan. Elsevier Academic Press. ISBN 978-0-08-102268-9.
- Rathinam NK, Samanta D, and Sani RK. 2017. Computational Nanotechnology – A tool for screening therapeutic nanomaterials against Alzheimer’s disease neuromethods: Computational modeling of drugs against Alzheimer’s disease. Ed. Roy K. Springer Nature, USA.
- Rathinam NK and Sani RK. 2017. Extremophilic Microbial Processes, In R. Sani and N.K. Rathinam. (eds) Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Bioenergy. Springer, Cham
- Rathinam NK, David A, and Sani RK. 2017. Fundamentals of Enzymatic Processes, In Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy. Ed. RK Sani and RN Krishnaraj. Springer, USA. Pg.5-29. ISBN 978-3-319-54684-1.
- Bibra M, Rathinam NK, and Sani RK. (2017) An Overview on Extremophilic Chitinases. In Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy, Ed. RK Sani and RN Krishnaraj. Springer, USA. Pg.225-247. ISBN 978-3-319-54684-1.
- Rathinam NK and R.K. Sani. 2017. Introduction to Extremozymes. In Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy. Ed. RK Sani and Krishnaraj RN. Springer, USA. Pg. 1-4. ISBN 978-3-319-54684-1.
- Kumar S, Bhalla A, Bibra M, and Sani RK. 2015. Thermophilic Biohydrogen Production: Challenges at the Industrial Scale. In Bioenergy: Opportunities and Challenges. Ed. R.N. Krishnaraj. Apple Academy Press, USA. pp 3-35.
- Bibra M, Wang J, Squillace P, Pinkelman R, Papendick S, Schneiderman S, Wood V, Amar V, Kumar S, Salem DR, and Sani RK. 2015. Biofuels and Value-added Products from Extremophiles. In Advances in Biotechnology. Eds. Neelu Nawani, Madhukar Khetmalas, Ashok Pandey, P. M Razdan and K. B Powar. I. K. International Publishing House Pvt. Ltd., Delhi. pp 17-51.
- Rastogi G and Sani RK. 2011. Molecular techniques to assess microbial community structure, function, and dynamics in the environment. Chapter in “Microbes and Microbial Technology: Agricultural and environmental applications”. Publisher Springer, USA. P 29-57.
Peer-Reviewed Publications
- Banerjee A, Sarkar S, Govil T, González-Faune P, Cabrera-Barjas G, Bandopadhyay R, Salem DR, and Sani RK. Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation. Frontiers in Microbiology. In Press.
- Laubach J, Joseph M, Brenza T, Gadhamshetty V, and Sani RK. 2021. Exopolysaccharide and biopolymer-derived films as tools for transdermal drug delivery. Journal of Controlled Release 329: 971–987. https://doi.org/10.1016/j.jconrel.2020.10.027 .
- Kumar A, Saini HS, Şengör SS, Sani RK, and Kumar S. 2021. Bioleaching of metals from waste printed circuit boards using bacterial isolates native to abandoned gold mine. BioMetals. https://doi.org/10.1007/s10534-021-00326-9. In Press.
- Govil T, Paste M, Samanta D, David A, Goh KM, Li X, Salem DR, and Sani RK. 2021. Metagenomics and culture dependent insights into the distribution of Firmicutes across two different sample types located in the Black Hill’s region of South Dakota, USA. Microorganisms. Jan 6;9(1):113. https://doi.org/10.3390/microorganisms9010113 .
- Vemuri B, Xia L, Chilkoor G, Kalimuthu J, Sani RK, Amarnath A, Kilduff J, and Gadhamshetty V. 2021. Municipal wastewater treatment and reuse with a bioelectrochemical module. Bioresource Technology. Volume 321, 124406. https://doi.org/10.1016/j.biortech.2020.124406 .
- Kalimuthu J, Dhiman S, Bedwell S, Vemuri B, Islam J, Sani RK and Gadhamshetty V. 2021. Electricity from methane by Methylococcus capsulatus Bath and Methylosinus trichosporium OB3b. Bioresource Technology. Volume 321, 124398. https://doi.org/10.1016/j.biortech.2020.124398 .
- Wang J, Salem DR, and Sani RK. 2021. Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp. WSUCF1: Characterization and bioactivities. New Biotechnology. 61:29-39. https://doi.org/10.1016/j.nbt.2020.11.004 .
- Wang J, Salem DR, and Sani RK. 2020. Synthesis of biopolymers from a Geobacillus sp. WSUCF1 using unprocessed corn stover. ACS Sustainable Chemistry & Engineering, 8, 25, 9483–9496. http://dx.doi.org/10.1021/acssuschemeng.0c02435..
- Shrestha N, Tripathi AK, Govil T, Sani RK, Urgun-Demirtas M, Kasthuri V and Gadhamshetty V. 2020. Electricity from lignocellulosic substrates by thermophilic Geobacillus species. Scientific Reports. 10, 17047. https://doi.org/10.1038/s41598-020-72866-y .
- Dar RA, Parmar M, Dar EA, Sani RK, and Phutela UG. 2021. Biomethanation of agricultural residues: Potential, limitations, and possible solutions. Renewable and Sustainable Energy Reviews 135 (2021) 110217. https://doi.org/10.1016/j.rser.2020.110217 .
- Bai Y, Rafiq MK, Lia S, Degen AA, Mašek O, Sun H, Han H, Wang T, Joseph S, Bachmann RT, Sani RK, Long R, and Shang Z. 2021. Biochar from pyrolyzed Tibetan Yak dung as a novel additive in ensiling sweet sorghum: an alternate to the hazardous use of yak dung as a fuel in the home. Journal of Hazardous Materials 403 (2021) 1236472. https://doi.org/10.1016/j.jhazmat.2020.123647 .
- Rathinam N, David A, and Sani RK. 2020. Bioelectrosynthesis technology for enhancing methane production using a thermophilic methanogenic consortium. Bioresource Technology. Volume 314, October 2020, 123892. https://doi.org/10.1016/j.biortech.2020.123892 .
- Govil T, Wang J, Samanta D, David A, Tripathi A, Rauniyar S, Salem DR, and Sani RK. 2020. Lignocellulosic feedstock: a review of a sustainable platform for cleaner production of nature’s plastics. Journal of Cleaner Production. Volume 270, 10 October 2020, 122521. https://doi.org/10.1016/j.jclepro.2020.122521. .
- Bibra M, Rathinam, NK, Johnson GR and Sani RK. 2020. Single pot biovalorization of food waste to ethanol by Geobacillus and Thermoanaerobacter spp. Renewable Energy. 155 1032-1041. https://doi.org/10.1016/j.renene.2020.02.093 .
- Govil T, Saxena P, Samanta D, Singh SS, Kumar S, Salem DR, and Sani RK. 2020. Adaptive enrichment of a thermophilic bacterial isolate for enhanced enzymatic activity. Microorganisms 2020, 8, 871; https://doi.org/10.3390/microorganisms8060871.
- Liew KJ, Bruce NC, Sani, RK, Chong, CS, Yaakop, AS, Shamsir MS, and Goh KM. 2020. Global transcriptomic responses of Roseithermus sacchariphilus strain RA in media added with beechwood xylan. Microorganisms 2020, 8, 976. https://doi.org/10.3390/microorganisms8070976 .
- David A, Tripathi AK, and Sani RK. 2020. Acetate Production from Cafeteria Wastes and Corn Stover Using a Thermophilic Anaerobic Consortium: A Prelude Study for the Use of Acetate for the Production of Value-Added Products. Microorganisms. 8 (3), 353. https://doi.org/10.3390/microorganisms8030353 .
- Tripathi AK, David A, Govil T, Rauniyar S, Rathinam NK, Goh KM, and Sani RK. 2020. Environmental Remediation of Antineoplastic Drugs: Present Status, Challenges, and Future directions. Processes, 8(7), 747; https://doi.org/10.3390/pr8070747 .
- Khan A, Salama ES, Chend Z, Nia H, Zhaoa S, Zhoua T, Peia Y, Sani RK, Linga Z, Liu P, and Li X. 2020. A novel biosensor for zinc detection based on microbial fuel cell system. Biosensors and Bioelectronics 147:111763. https://doi.org/10.1016/j.bios.2019.111763 .
- Islam J, Chilkoor G, Kalimuthu J, Dhiman S, Sani RK, and Gadhamshetty V. 2020. Vitamin-C-enabled reduced graphene oxide chemistry for tuning biofilm phenotypes of methylotrophs on nickel electrodes in microbial fuel cells. Bioresourse Technology. 300: 122642. https://doi.org/10.1016/j.biortech.2019.122642 .
- Rathinam NK, Gorky, Bibra M, Salem DR, and Sani RK. 2020. Bioelectrochemical approach for enhancing lignocellulose degradation in Geobacillus strain WSUCF1, a thermophile. Bioresourse Technology. 295: 122271. https://doi.org/10.1016/j.biortech.2019.122271 .
- Choudhary A, Kumar A, Govil T, Sani RK, Dutt G and Kumar S. 2020. Sustainable production of biogas in a large bioreactor under psychrophilic and mesophilic conditions. Journal of Environmental Engineering. 146(3): 04019117. https://doi.org/10.1061/(ASCE)EE.1943-7870.0001645 .
- Govil T. Sharma W, Chauhan N, Kumar S, Salem RD, and Sani RK. 2019. MINES Method for Genomic DNA Extraction from Deep Biosphere Biofilms. Journal of Microbiological Methods. 167: 105730. https://doi.org/10.1016/j.mimet.2019.105730
- Rai R, Bibra M, Chadha BS, and Sani RK. 2019. Enhanced hydrolysis of lignocellulosic biomass with doping of a highly thermostable recombinant laccase. International Journal of Biological Macromolecules. 137: 232-237. https://doi.org/10.1016/j.ijbiomac.2019.06.221 .
- Wang J, Goh KM, Salem DR, and Sani RK. 2019. Genome analysis of a thermophilic exopolysaccharide-producing bacterium – Geobacillus sp. WSUCF1. Scientific Reports – Nature. Feb 7;9(1):1608. https://doi.org/10.1038/s41598-018-36983-z .
- Wang J, Salem DR, and Sani RK. 2019. Extremophilic exopolysaccharides: A review and new perspectives on engineering strategies and applications. Carbohydrate Polymers 205: 8–26. https://doi.org/10.1016/j.carbpol.2018.10.011 .
- Rathinam NK, Bibra M, Rajan Magesh, Salem DR, and Sani RK. 2019. Thermophiles for Biohydrogen Production in Microbial Electrolytic Cells. Bioresource Technology. 277: 171-178. https://doi.org/10.1016/j.biortech.2019.01.020 .
- Liew KJ, Ngooi CY, Shamsir MS, Sani RK, Chong CS, and Goh KM. 2019. Heterologous expression, purification and biochemical characterization of a new endo-1,4-β-xylanase from Rhodothermaceae bacterium RA. Protein Expression and Purification. Aug 1; 164:105464. https://doi.org/10.1016/j.pep.2019.105464 .
- Rathinam NK, Gorky, Bibra M, Salem DR, and Sani RK. 2019. Short Term Atmospheric Pressure Cold Plasma treatment: A Novel Strategy for enhancing the Substrate Utilization in a thermophile, Geobacillus sp. strain WSUCF1. Bioresource Technology. 278: 477-480. https://doi.org/10.1016/j.biortech.2019.01.028 .
- Donati ER, Sani RK, Goh KM, and Chan KG. 2019. Recent Advances in Bioremediation/Biodegradation by Extreme Microorganisms. Frontiers in Microbiology. 10: 1851. https://doi.org/10.3389/fmicb.2019.01851 .
- Basotra N, Dhiman SS, Agrawal D. Tsange A, and Sani RK. 2019. Characterization of a novel Lytic Polysaccharide Monooxygenase from Malbranchea cinnamomea exhibiting dual catalytic behavior. Carbohydrate Research 478: 46-53. https://doi.org/10.1016/j.carres.2019.04.006 .
- Chilkoor G, Karanam S, Star S, Shrestha N, Sani RK, Upadhyayula V, Ghoshal D, Koratkar N, Meyyappan M, and Gadhamshetty V. 2018. Hexagonal Boron Nitride: The Thinnest Insulating Barrier to Microbial Corrosion. ACS Nano. 12(3): 2242-2252.
- Rathinam NK, Tripathi AK, Smirnova AL, Beyenal H, and Sani RK. 2018. Engineering Rheology of Electrolytes using Agar for Improving the Performance of Bioelectrochemical Systems. Bioresource Technology. 263: 242-249.
- Dhiman SS, Shrestha N, David A, Basotra N, Johnson, GR, Chadha BS, Gadhamshetty V, and Sani RK. 2018. Producing methane, methanol, and electricity from organic waste of fermentation reaction using novel microbes. Bioresource Technology. 258: 270-278.
- Wang J, Bibra M, Venkateswaran K, Salem DR, Rathinam NK, Gadhamshetty V, and Sani RK. 2018. Biohydrogen Production from Space Crew’s Waste Simulants using Thermophilic Consolidated Bioprocessing. Bioresource Technology. 255: 349-353.
- Shrestha N, Chilkoor G, Vemuri B, Rathinam NK, Sani RK, and Gadhamshetty V. 2018. Extremophiles for microbial-electrochemistry applications: A critical review. Bioresource Technology. 255: 318-330.
- Bibra M, Kumar S, Wang J, Bhalla A, Salem DR, and Sani RK. 2018. Single Pot Bioconversion of Prairie Cordgrass into Biohydrogen by Thermophiles. Bioresource Technology. 18: 266:232-241. .
- David A, Govil T, Tripathi, AK, McGeary, J, Farrar K, and Sani RK. 2018. Thermophilic Anaerobic Digestion: Enhanced and Sustainable Methane Production from Co-Digestion of Food and Lignocellulosic Wastes. Energies. 11, 2058.
- Bibra M, Kunreddy VR, and Sani RK. 2018. Thermostable Xylanase Production by Geobacillus sp. strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass. Microorganisms. 6(3), 93..
- Liew KJ, Teo SC, Shamsir MS, Sani RK, Chong CS, Chan KG, and Goh KM. 2018. Complete Genome Sequence of Rhodothermaceae bacterium RA with Cellulolytic and Xylanolytic Activity. 3Biotech (2018) 8:37.
- Sharma NK, Behera S, Arora R, Kumar S, and Sani RK. 2018. Xylose transport in yeast for lignocellulosic ethanol production: Current status. Journal of Bioscience and Bioengineering. 125 (3), 259-267.
- Rathinam NK, Berchmans S, Salem D, Sani RK. 2018. Rewiring the Microbe-Electrode Interfaces with Biologically Reduced Graphene Oxide for Improved Bioelectrocatalysis. Bioresource Technology. 256: 195-200.
- Carlson C, Singh NK, Bibra M, Sani RK and Venkateswaran K. 2018. Molecular microbial community analysis of extremophilic environments harboring UVC254-resistant strains of Geobacillus and Bacillus. Applied Microbiology and Biotechnology. 102:1869-1887.
- Dhiman SS, David A, Braband VW, Hussein A, Salem DR, and Sani RK. 2017. Improved bioethanol production from corn stover: Role of enzymes, inducers and simultaneous product recovery. Applied Energy. 208: 1420-1429.
- Dhiman SS, David A, Shrestha N, Johnson GR, Benjamin KM, Gadhamshetty V, and Sani RK. 2017. Simultaneous hydrolysis and fermentation of unprocessed food waste into ethanol using thermophilic anaerobic bacteria. Bioresource Technology. 244(1): 733-740.
- Sani RK. 2017. Extremophilic microbes enable new methane pathways. Chemical Engineering Magazine. November 2017. Page 10. https://www.chemengonline.com/extremophilic-microbes-enable-new-methane-pathways/ .
- Goh KM, Chan KG, Sani RK, Donati E, Reysenbach AL. 2017. Genetics, genomics and –omics of thermophiles. Frontiers in Microbiology. 8: 560.
- Singh NK, Carlson C, Sani RK, and Venkateswaran K. 2017. Draft genome sequences of thermophiles isolated from Yates Shaft, a deep-subsurface environment. Genome Announcements*. 5(22). .
- Krishnaraj RN, Samanta D, Kumar A, and Sani RK. 2017. Bioprospecting of the thermostable cellulolytic enzyme through modeling and virtual screening method. Canadian journal of Biotechnology*. 1:19-25..
- Chan CS, Sin LL, Chan KG, Shamsir MS, Manan FA, Sani RK, and Goh KM. 2016. Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1. Biotechnology for Biofuels. 9(1):174.
- Şengör SS, Singh G, Dohnalkova A, Spycher N, Ginn TR, Peyton BM, and Sani RK. 2016. Impact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20. BioMetals. 29(6): 965-980.
- Bhalla A, Bischoff K, and Sani RK. 2015. Highly thermostable xylanase production from a thermophilic Geobacillus sp. strain WSUCF1 utilizing lignocellulosic biomass. Frontiers in Bioengineering and Biotechnology. 3:84.
- Bhalla A, Bischoff KM, Uppugundla N, Balan V, and Sani RK. 2014. Novel Thermostable Endo-xylanase Cloned and Expressed from Bacterium Geobacillus sp. WSUCF1. Bioresourse Technology. 165: 314–318.
- Bhalla A, Bischoff KM, and Sani RK. 2014. Highly thermostable GH39 β-xylosidase from a Geobacillus sp. strain WSUCF1. BMC Biotechnology. 14:963.
- Singh G, Şengör SS, Bhalla A, Kumar S, De J, Stewart B, Spycher N, Ginn T, Peyton B, and Sani RK. 2014. Reoxidation of Biogenic Reduced Uranium – A Challenge Towards Bioremediation. Critical Reviews in Environmental Science and Technology. 44(4): 391-415.
- Bhalla A, Kainth A, and Sani RK. 2013. Draft Genome Sequence of Lignocellulose Degrading Thermophilic Bacterium Geobacillus sp. strain WSUCF1. Genome Announcements. 1(4):e00595-13. doi:10.1128/genomeA.00595-13.
- Rastogi G, Gurram RN, Bhalla A, Jaswal R, Gonzalez R, Bischoff KM, Hughes SR, Sudhir K, and Sani RK. 2013. Fermentation of glucose, xylose, and glycerol by bacteria isolated from the extreme biosphere of the former Homestake gold mine, South Dakota. Journal of Frontiers in Microbiotechnology, Ecotoxicology and Bioremediation. 4: 1-8.
- Bhalla A, Bansal N, Kumar S, and Sani RK. 2013. Improved Lignocellulose Conversion to Biofuels with Thermophilic Bacteria. Bioresource Technology. 128: 751–759.
- Stewart BD, Girardot C, Spycher N, Sani RK and Peyton BM. 2013. Influence of Chelating Agents on Biogenic Uraninite Reoxidation by Fe(III) (Hydr)oxides. Environmental Science and Technology. 47(1): 364-371.
- Kumar S, Bhalla A, Shende RV, and Sani RK. 2012. Decentralized Thermophilic Biohydrogen: A More Efficient and Cost-Effective Process. BioResources. 7(1), 1-2..
- Spycher NF, Issarangkun M, Stewart B, Sengor S, Belding E, Ginn, TM, Peyton BM, and Sani RK. 2011. Biogenic Uraninite Precipitation and its Reoxidation by Iron(III)(Hydr)oxides: A Reaction Modeling Approach. Geochim. Cosmochim. Acta. 75: 4426-4440. .
- Sivaswamy V, Peyton BM, Viamajala S, Gerlach R, Apel WA, Sani RK, Dohnalkova A, and Borch T. 2011. Multiple Mechanisms of Uranium Immobilization by Cellulomonas sp. strain ES6. Biotechnology and Bioengineering. 108(2):264-276. .
- Rastogi G, Barua S, Sani RK, and Peyton BM. 2011. Investigation of Microbial Populations in the Extremely Metal-Contaminated Coeur d’ Alene River Sediments. Microbial Ecology. 62(1): 1-13. .
- Zambare VP, Bhalla A, Muthukumarappan K, Sani RK, and Christopher LP. 2011. Bioprocessing of Agricultural Residues to Ethanol Utilizing a Cellulolytic Extremophile. Extremophiles.15: 611-618. .
- Rastogi G, Osman S, Kukkadapu R, Engelhard M, Vaishampayan PA, Andersen GL, and Sani RK. 2010. Microbial and Mineralogical Characterizations of Soils Collected from the Deep Biosphere of the Former Homestake Gold Mine, South Dakota. Microbial Ecology. 60: 539-550. .
- Moberly JG, Staven A, Sani RK, Peyton BM. 2010. Influence of pH and Inorganic Phosphate on Toxicity of Zinc to Arthrobacter sp. Isolated from Heavy-Metal-Contaminated Sediments. Environmental Science and Technology. 44: 7302-7308. .
- Rastogi G, Bhalla A, Adhikari A, Bischoff KM, Hughes SR, Christopher LP, and Sani RK. 2010. Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains. Bioresource Technology. 101: 8798–8806. .
- Waddell EJ, Elliott TJ, Vahrenkamp JM, Roggenthen WM, Sani RK, Anderson CM, and Bang SS. 2010. Phylogenetic evidence of noteworthy microflora from the subsurface of the former Homestake gold mine, Lead, South Dakota. Environmental Technology. 31: 979–991. .
- Sani RK, Rastogi G, Moberly JG, Dohnalkova A, Ginn TR, Spycher N, Shende RV, and Peyton BM. 2010. The toxicity of lead to Desulfovibrio desulfuricans G20 in the presence of goethite and quartz. Journal of Basic Microbiology. 50: 160-170. .
- Rastogi G, Osman S, Andersen GL, and Sani RK. 2010. Microbial diversity in uranium mining-impacted soils as revealed by high-density 16S microarray and clone library. Microbial Ecology. 59(1): 94-108. .
- Rastogi G, Muppidi GL, Gurram RN, Adhikari A, Bischoff KM, Hughes SR, Apel WA, Bang SS, Dixon DJ, and Sani RK. 2009. Isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the Homestake gold mine, Lead, South Dakota, USA. Journal of Industrial Microbiology & Biotechnology. 36:585-598. .
- Moberly JG, Borch T, Sani RK, Spycher NF, Şengör SS, Ginn TR, and Peyton BM. 2009. Heavy metal-mineral associations in Coeur d’Alene river sediments: A synchrotron-based analysis. Water, Air, and Soil Pollution. 200: 195-208. .
- Şengör SS, Barua S, Gikas P, Ginn TR, Peyton BM, Sani RK, and Spycher NF. 2009. Influence of Heavy Metals on Microbial Growth Kinetics Including Lag Time: Mathematical Modeling and Experimental Verification. Environmental Toxicology and Chemistry. 28: 2020–2029. .
- Rastogi G, Stetler LD, Peyton BM, and Sani RK. 2009. Molecular analysis of prokaryotic diversity in the deep subsurface of the Homestake gold mine, SD, USA. The Journal of Microbiology. 47: 371-384. .
- Rastogi G, Sani RK, Peyton BM, Moberly JG, and Ginn TR. 2009. Molecular studies on the microbial diversity associated with mining-impacted Coeur d’ Alene river sediments. Microbial Ecology. 58:129-139. .
- Sani RK, Peyton BM, and Dohnalkova A. 2008. Comparison of uranium(VI) removal by Shewanella oneidensis MR-1 in flow and batch reactors. Water Research. 42(12): 2993-3002. .
- Viamajala S, Smith WA, Sani RK, Peyton BM, Apel WA, Neal AL, and Petersen JN. 2007. Isolation and characterization of Cr(VI) reducing Cellulomonas spp. from subsurface soils: Implications for long-term chromate reduction. Bioresource Technology. 98(3): 612-622..
- Cummings DE, Fendorf S, Singh N, Sani RK, Peyton BM, and Magnuson TS. 2007. Reduction of Cr(VI) under acidic conditions by the facultative Fe(III)-reducing bacterium Acidiphilium cryptum. Environmental Science and Technology. 41(1): 146-152. .
- Sengör SS, Spycher N, Ginn TR, Moberly JE, Peyton BM, and Sani RK. 2007. Reductive dissolution and metal transport in Lake Coeur d’Alene sediments. In Water-Rock Interaction, WRI-12 (Bullen T. and Wang Y., eds.), vol. 2. Taylor & Francis Group, London, pp. 895-899..
- Sengör SS, Spycher N, Ginn TR, Sani RK, and Peyton BM. 2007. Biogeochemical reactive-diffusive transport of heavy metals in Lake Coeur d’Alene sediments. Applied Geochemistry. 22 (12): 2569-2594. .
- Sani RK, Peyton BM, and Dohnalkova A. 2006. Toxic effects of uranium on Desulfovibrio desulfuricans G20. Environmental Toxicology and Chemistry. 25: 2131-1238..
- Sani RK, Peyton BM, Amonette JE, and Dohnalkova A. 2005. Reoxidation of uranium in the presence of iron(III)-(hydr)oxides under sulfate reducing conditions. Environmental Science and Technology. 39: 2059-2066. .
- Sani RK, Peyton BM, Amonette JE, and Geesey GG. 2004. Reduction of uranium(VI) under sulfate reducing conditions in the presence of Fe(III)-(hydr)oxides. Geochimica et Cosmochimica Acta. 68: 2639-2648. .
- Beyenal H, Sani RK, Peyton BM, Dohnalkova A, Amonette JE, and Lewandowski Z. 2004. Uranium immobilization by sulfate reducing biofilms. Environmental Science and Technology. 38: 2067-2074. .
- Viamajala S, Peyton BM, Sani RK, Apel WA, and Petersen JN. 2004. Toxic effects of Cr(VI) on anaerobic and aerobic growth of Shewanella oneidensis MR-1. Biotechnology Progress. 20: 87-95. .
- Sani RK, Peyton BM, and Jandhyala M. 2003. Toxicity of lead in aqueous medium to desulfovibrio desulfuricans G20. Environmental Toxicology and Chemistry. 22: 252-260..
- Sani RK. 2003. Bioremediation of contaminated soils, sediments, and groundwater at US department of energy facilities: with possible scientific solutions – A NABIR Program. Soul of Science, Newsletter of INSCR, ISSN: 0972-4915, 3: 6-7..
- Chakraborti S, Sani RK, Sahoo DK, Banerjee UC, and Sobti RC. 2003. Production and partial characterization of a novel -galactosidase from a newly isolated Bacillus polymyxa. Scientia Iranica. 10: 279-286. .
- Sani RK, Peyton BM, Smith WA, Apel WA, and Petersen JN. 2002. Dissimilatory reduction of Cr(VI), Fe(III), and U(VI) by Cellulomonas isolates. Applied Microbiology and Biotechnology. 60: 192-199. .
- Sani RK, Peyton BM, and Brown LT. 2001. Copper-induced inhibition in growth of Desulfovibrio desulfuricans G20: assessment of its toxicity and correlation with zinc and lead. Applied and Environmental Microbiology, 67: 4765-4772. .
- Sani RK, Geesey GG, and Peyton BM. 2001. Assessment of lead toxicity to Desulfovibrio desulfuricans G20: influence of components of Lactate C medium. Advances in Environmental Research. 5: 269-276. .
- Chakraborti S, Sani RK, Banerjee UC, and Sobti RC. 2000. Purification and characterization of a novel β-galactosidase from Bacillus sp MTCC 3088. Journal of Industrial Microbiology and Biotechnology. 24: 58-63. .
- Sani RK and Banerjee UC. 1999. Decolorization of triphenylmethane dyes and textile and dye-stuff effluents by Kurthia sp. Enzyme and Microbial Technology. 24: 433-437. .
- Sani RK and Banerjee UC. 1999. Decolorization of acid green 20, a textile dye by white rot fungus, Phanerochaete chrysosporium in low cost medium. Advances in Environmental Research. 2: 485-490. .
- Sani RK, Jolly RS, and Banerjee UC. 1999. Reduction of gentian violet to leucogentian violet by Kurthia sp. and assessment of toxicity. Advances in Environmental Research. 3: 368-377..
- Sani RK and Banerjee UC. 1999. Screening for organisms applicable to the decolorization of triphenylmethane dyes and optimization of biotransformation conditions in stirred tank reactor. Indian Journal of Environment & Ecoplanning. 2(1): 1-9..
- Sani RK, Chakraborti S, Sobti RC, Patnaik PR, and Banerjee UC. 1999. Characterization and some reaction engineering aspects of thermostable extra-cellular -galactosidase from Bacillus sp. Folia Microbiologica, 44: 367-371. .
- Mohapatra BR, Sani RK, and Banerjee UC. 1999. Characterization of L-asparaginase from Bacillus sp. isolated from an intertidal marine alga (Sargassum sp.). Letters in Applied Microbiology. 21: 380-383. .
- Banerjee UC, Sani RK, Azmi W, and Soni R. 1999. Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive. Process Biochemistry. 35: 213-219..
- Sani RK and Banerjee UC. 1998. Comparison of static and shake culture in the decolorization of textile dyes and dye effluent by Phanerochaete chrysosporium. Folia Microbiologica. 43: 85-88. .
- Azmi W, Sani RK, and Banerjee UC. 1998. Biodegradation of triphenylmethane dyes. Enzyme and Microbial Technology. 22: 185-191. .
Patent and Invention Disclosures
Sani RK, Azmi W, and Banerjee UC. 1997. A process for the preparation of decolorizing agent useful for the decolorization of triphenylmethane dyes. Patent No. NF 6/DEL/97.
- Title of Invention: Biopolymer Films for Drug Delivery. Inventors: Sani RK and Laubach J. April 2021. We have disclosed this invention to the scientific community/SDSM&T Research Affairs…
- Title of Invention: Methods for Regulating the Inflammatory Response of Cytokines in Response to Cold Atmospheric Plasma Treatment. Inventors: Diwakar P, Bright T, Brenza T, and Sani RK. March 2021. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Biopolymers from untreated lignocellulosic biomass using a new thermophilic Geobacillus sp. Inventors: Govil T, Salem DR, and Sani RK. October 2018. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Sequential Thermophilic Microbial bioprocess for Bioethanol and Byproducts (STEMBnB). Inventors: Bibra M and Sani RK. October 2018. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Biopolymers produced by thermophilic strain Geobacillus sp. WSUCF1 using untreated corn stover. Inventors: Wang J, Salem DR, and Sani RK. February 2017. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Conversion of Raw and Untreated Disposal into Ethanol (CRUDE). Inventors: Sani RK and Dhiman S. May 2016. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Novel Thermostable Endo-xylanase from a Bacterium Geobacillus sp. strain WSUCF1. Inventors: Rajesh Sani, Aditya Bhalla, and Mohit Bibra. May 2016: We have disclosed this invention to the scientific community/SDSM&T Research Affairs. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Novel Thermostable β-xylosidase from a Bacterium Geobacillus sp. strain WSUCF1. Inventors: Rajesh Sani, Aditya Bhalla, and Mohit Bibra. May 2014. We have disclosed this invention to the scientific community/SDSM&T Research Affairs..
- Title of Invention: Thermostable hemicellulases from Geobacillus sp. strain WSUCF1. Inventors: Rajesh Sani, Aditya Bhalla, and Mohit Bibra. May 2014: We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Biopolymers from untreated lignocellulosic biomass using a new thermophilic Geobacillus sp. Inventors: Govil T, Salem DR, and Sani RK. October 2018. We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Sequential Thermophilic Microbial bioprocess for Bioethanol and Byproducts (STEMBnB). Inventors: Bibra M and Sani RK. October 2018. We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Biopolymers produced by thermophilic strain Geobacillus WSUCF1 using untreated corn stover. Inventors: Wang J, Salem DR, and Sani RK. February 2017. We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Conversion of Raw and Untreated Disposal into Ethanol (CRUDE). Inventors: Sani RK and Dhiman S. May 2016. We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Novel Thermostable Endo-xylanase from a Bacterium Geobacillus strain WSUCF1. Inventors: Rajesh Sani, Aditya Bhalla, and Mohit Bibra. May 2015: We have disclosed this invention to the scientific community/SDSM&T Research Affairs. We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Novel Thermostable β-xylosidase from a Bacterium Geobacillus strain WSUCF1. Inventors: Rajesh Sani, Aditya Bhalla, and Mohit Bibra. May 2014. We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
- Title of Invention: Thermostable hemicellulases from Geobacillus strain WSUCF1. Inventors: Rajesh Sani, Aditya Bhalla, and Mohit Bibra. May 2014: We have disclosed this invention to the scientific community/SDSM&T Research Affairs.
Books Edited
Dr. Sani has edited following ten books. These texts are currently being used for Biol 431/531: Industrial Microbiology (ABS new course which will be developed by me) and CBE 741: Microbial and Enzymatic Processing.

- Microbial Interactions at Nanobiotechnology Interfaces: Molecular Mechanisms and Applications. August 2021. Editors: Rathinam NK and Sani RK. WILEY. ISBN: 9781119617198
- Biofilm Engineering. August 2019. Editors: Rathinam NK and Sani RK. American Chemical Society (ACS). Publication Date (Web): ISBN 9780841234710 (https://doi.org/10.1021/bk-2019-1323)
- Next Generation Biomanufacturing Technologies. Publication Date (Web): December 3, 2019. Editors: Rathinam NK and Sani RK. American Chemical Society. ISBN13: 9780841235007. DOI: 10.1021/bk-2019-1329. https://pubs.acs.org/doi/book/10.1021/bk-2019-1329
- Bioelectrochemical Interface Engineering. September 2019. Editors: Rathinam NK and Sani RK. WILEY. ISBN: ISBN: 978-1-119-53854-7. https://www.wiley.com/en-us/Bioelectrochemical+Interface+Engineering-p-9781119538547
- Biovalorisation of Wastes to Renewable Chemicals and Biofuels. 2019. Editors: Rathinam NK and Sani RK. Elsevier. ISBN: 9780128179512. October 2019. https://www.elsevier.com/books/biovalorisation-of-wastes-to-renewable-chemicals-and-biofuels/krishnaraj/978-0-12-817951-2
- Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy. 2017. Editors: Sani RK and Rathinam NK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. ISBN 978-3-319-54684-1. http://www.springer.com/gp/book/9783319546834
- Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. 2018. Editors: Sani RK and Rathinam NK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. ISBN 978-3-319-74459-9. http://www.springer.com/us/book/9783319744575
- Biorefining of Biomass to Biofuels: Opportunities and Perception Biofuel and Biorefinery Technologies Series. 2018. Editors: Kumar S and Sani RK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. ISBN 978-3-319-67678-4. http://www.springer.com/us/book/9783319676777
- Proceedings of the Second International Conference on Recent Advances in Bioenergy Research. Springer Proceedings in Energy. 2018. Editors: Kumar S, Sani RK, and Yadav YK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. ISBN 978-981-10-6107-3. http://www.springer.com/us/book/9789811061066
- Genetics, Genomics and – Omics of Thermophiles. 2017. Editors: Goh KM, Chan KG, Sani RK, Dona ER, and Reysenbach AL. Publisher: Frontiers Media SA. ISBN: 9782889452019. Product Name: Frontiers Research Topic E-book. http://www.frontiersin.org/books/Genetics_Genomics_and_Omics_of_Thermophiles/1223
- Biofilm Engineering. Editors: Rathinam NK and Sani RK. American Chemical Society.
- Bioelectrochemical Interface Engineering. Editors: Rathinam NK and Sani RK. WILEY. ISBN: 978-1-119-53854-7, October 2019. https://www.wiley.com/en-us/Bioelectrochemical+Interface+Engineering-p-9781119538547
- Biovalorisation of Wastes to Renewable Chemicals and Biofuels. Editors: Rathinam NK and Sani RK. Elsevier. ISBN: 9780128179512. October 2019. https://www.elsevier.com/books/biovalorisation-of-wastes-to-renewable-chemicals-and-biofuels/krishnaraj/978-0-12-817951-2
- Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. Editors: Sani RK and Rathinam NK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. In Press. http://www.springer.com/us/book/9783319744575
- Biorefining of Biomass to Biofuels: Opportunities and Perception Biofuel and Biorefinery Technologies Series. Editors: Kumar S and Sani RK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. In Press. http://www.springer.com/us/book/9783319676777
- Proceedings of the Second International Conference on Recent Advances in Bioenergy Research. Springer Proceedings in Energy. Editors: Kumar S, Sani RK, and Yadav YK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. In Press. http://www.springer.com/us/book/9789811061066
- Genetics, Genomics and – Omics of Thermophiles. 2017. Editors: Goh KM, Chan KG, Sani RK, Dona ER, and Reysenbach AL. Publisher: Frontiers Media SA. ISBN: 9782889452019. Product Name: Frontiers Research Topic E-book. http://www.frontiersin.org/books/Genetics_Genomics_and_Omics_of_Thermophiles/1223
- Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy. 2017. Editors: Sani RK and Rathinam NK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. ISBN 978-3-319-54684-1 http://www.springer.com/gp/book/9783319546834
- Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy. 2017. Editors: Sani RK and Rathinam NK. Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland. ISBN 978-3-319-54684-1. http://www.springer.com/gp/book/9783319546834






Our Lab Manuals
A lab manual has been published in 2019
Bibra M, Govil T, and Sani RK. 2019. Biochemistry Laboratory (Chem 464L/564L). Science Lab Studios (http://www.sciencelabstudios.com/sani01)